The role of DNA repair on cell killing by charged particles.

It can be noted that it is not simple double strand breaks (dsb) but the non-reparable breaks that are associated with high biological effectiveness in the cell killing effect for high LET radiation. Here, we have examined the effectiveness of fast neutrons and low (initial energy = 12 MeV/u) or high (135 MeV/u) energy charged particles on cell death in 19 mammalian cell lines including radiosensitive mutants. Some of the radiosensitive lines were deficient in DNA dsb repair such as LX830, M10, V3, and L5178Y-S cells and showed lower values of relative biological effectiveness (RBE) for fast neutrons if compared with their parent cell lines. The other lines of human ataxia-telangiectasia fibroblasts, irs 1, irs 2, irs 3 and irs1SF cells, which were also radiosensitive but known as proficient in dsb repair, showed moderated RBEs. Dsb repair deficient mutants showed low RBE values for heavy ions. These experimental findings suggest that the DNA repair system does not play a major role against the attack of high linear energy transfer (LET) radiations. Therefore, we hypothesize that a main cause of cell death induced by high LET radiations is due to non-reparable dsb, which are produced at a higher rate compared to low LET radiations.     

Simulation experiments of the effect of space environment on bacteriophage and DNA thin films.

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.     

Influence of long-term altered gravity on the swimming performance of developing cichlid fish: including results from the 2nd German Spacelab Mission D-2.

This study presents qualitative and quantitative data concerning gravity-dependent changes in the swimming behaviour of developing cichlid fish larvae (Oreochromis mossambicus) after a 9 resp. 10 days exposure to increased acceleration (centrifuge experiments), to reduced gravity (fast-rotating clinostat), changed accelerations (parabolic aircraft flights) and to near weightlessness (2nd German Spacelab Mission D-2). Changes of gravity initially cause disturbances of the swimming performance of the fish larvae. With prolonged stay in orbit a step by step normalisation of the swimming behaviour took place in the fish. After return to 1g earth conditions no somersaulting or looping could be detected concerning the fish, but still slow and disorientated movements as compared to controls occurred. The fish larvae adapted to earth gravity within 3-5 days. Fish seem to be in a distinct early developmental stages extreme sensitive and adaptable to altered gravity; However, elder fish either do not react or show compensatory behaviour e.g. escape reactions.     

Synthetic-aperture radar processing using fast factorized back-projection

Exact synthetic aperture radar (SAR) inversion for a linear aperture may be obtained using fast transform techniques. Alternatively, back-projection integration in time domain can also be used. This technique has the benefit of handling a general aperture geometry. In the past, however, back-projection has seldom been used due to heavy computational burden. We show that the back-projection integral can be recursively partitioned and an effective algorithm constructed based on aperture factorization. By representing images in local polar coordinates it is shown that the number of operations is drastically reduced and can be made to approach that of fast transform algorithms. The algorithm is applied to data from the airborne ultra-wideband CARABAS SAR and shown to give a reduction in processing time of two to three orders of magnitude.     

Microdosimetric measurements of heavy ion tracks.

The biological effectiveness of radiations depends on the spatial pattern of ionizations and excitations produced by the charged particle tracks involved. Ionizations produced by both the primary ion and by energetic delta rays may contribute to the production of biologically relevant damage and to the concentration of damage which may effect the probability of repair. Although average energy concentration (dose) can be calculated using homogeneous track models, the energy is actually concentrated in small volumes containing segments of the ion and delta ray tracks. These local concentrations are studied experimentally using low pressure proportional counters, and theoretically, using Monte Carlo methods. Small volumes near an ion track may be traversed by a delta ray. If they are, the energy deposited will be similar to that produced by a single electron track in a low-energy x-ray irradiation. The probability of a delta ray interaction occurring decreases with the square of the radial distance from the track. The average energy deposited is the product of this probability and the energy deposited in an interaction. Average energy deposited calculated from measured interaction probability is in good agreement with the results of homogeneous track models.     

An experiment with an S-band radar using pulse compression andrange sidelobe suppression for meteorological measurements

A major technology barrier to the application of pulse compression for the meteorological functions required by a next generation ATC radar is range/time sidelobes which mask and corrupt observations of weak phenomena occurring near areas of strong extended meteorological scatterers. Techniques for suppressing range sidelobes are well known but without prior knowledge of the scattering medium's velocity distribution their performance degrades rapidly in the presence of Doppler. Recent investigations have presented a “doppler tolerant” range sidelobe suppression technique. The thrust of the work described herein is the extension of previous simulations to actual transmitted dispersed/coded waveforms using the S-band surveillance radar located at Rome Laboratory Surveillance Facility. The objectives of the experiment are: 1) to extend the verification of the simulation of the Doppler tolerant technique; and 2) to demonstrate that the radar transmitter, waveform generator, and receiver imperfections do not significantly degrade resolution, performance or reliability of meteorological spectral moment estimates     

Application of Gram-Schmidt algorithm to fully adaptive arrays

The Gram-Schmidt orthogonalization (GSO) algorithm has excellent numerical performance and is readily applicable to systolic implementations such as in a field of adaptive cancellation systems. A modified GSO algorithm for a fully adaptive array is proposed and computer simulations show that the proposed algorithm gives superior performance. A systolic implementation of the proposed GSO algorithm for fully adaptive array is presented. A feedback mode GSO algorithm for use with analog weights is also presented and has been shown to have excellent performance in the presence of weight errors     

Automated video protection, monitoring & detection

The latest generation of computer vision technology is revolutionizing concepts, applications, and products in video surveillance and CCTV. This is of prime relevance to security for large outdoor facilities such as commercial airfields, refineries, power plants, and office/industrial campuses. Most airfields, for example, have open (unfenced) perimeters, high volume heterogeneous traffic, are easily accessed on foot or by water, and exist in areas where regulations providing a safety buffer are difficult to legislate or enforce. And all airfields require 24/7 outdoor monitoring - snow, fog, rain, or shine. Likewise, most high-value facilities appealing to criminals and terrorists are in close proximity to public areas (roads, residences, city, etc.). The appeal of automated real-time surveillance is obvious $maximizing efficiency and effectiveness of security personnel and resources while increasing the probability of preventing a serious security breach. Computer vision based solutions have the potential for very discriminating detection and very low false alarms. The bottom line is that applied computer vision has the potential for the greatest return on investment (ROI), both short-term and long-term.     

Solar and Heliospheric Modulation of Galactic Cosmic Rays

The significance of external influences on the environment of Earth and its atmosphere has become evident during recent years. Especially, on time scales of several hundred years, the cosmogenic isotope concentration during the Wolf-, Spoerer-, Maunder- and Dalton-Minimum indicates an increased cosmic ray flux. Because these grand minima of solar activity coincide with cold periods, a correlation of the Earth climate with the cosmic ray intensities is plausible. Any quantitative study of the effects of energetic particles on the atmosphere and environment of the Earth must address their transport to Earth and their interactions with the Earth’s atmosphere including their filtering by the terrestrial magnetosphere. The first problem is one of the fundamental problems in modern cosmic ray astrophysics, and corresponding studies began in the 1960s based on Parker’s cosmic ray modulation theory taking into account diffusion, convection, adiabatic deceleration, and (later) the drift of energetic particles in the global heliospheric magnetic field. It is well established that all of these processes determining the modulation of cosmic rays are depending on parameters that are varying with the solar magnetic cycle. Therefore, the galactic cosmic ray intensities close to Earth is the result of a complex modulation of the interstellar galactic spectrum within the heliosphere. The modern view of this cosmic ray modulation is summarized in our contribution.     

How Operational Oceanography Can Benefit from Dynamic Topography Estimates as Derived from Altimetry and Improved Geoid

With a precise geoid, GOCE will allow an estimation of absolute dynamic topography from altimetry. The projected benefits to operational oceanography and its applications are analyzed herein. After a brief overview of operational oceanography, we explain how the new geoids will be used in the future to improve real time altimeter products and to better constrain modelling and data assimilation systems. A significant impact is expected both for mesoscale (e.g. better estimations and forecasts of currents for pollution monitoring, marine safety, offshore industry) and climate (better initialization of coupled ocean/atmosphere models) applications. This revised version was published online in August 2006 with corrections to the Cover Date.